Technologies

time icon Aug. 14, 2019

Selective Apoptotic Induction in Cancer Cells

Technology description

In cancer, the signaling cascade for programmed cell death, apoptosis, is often mutated leading to resistance from natural pro-death signals. PAC-1 and related compounds are small molecules capable of specifically killing cancer cells by inducing apoptosis. It does so by targeting an inactive apoptotic precursor, procaspase-3, that is up regulated in many cancer types. In vitro and in vivo studies have shown PAC-1 to be efficacious in reducing tumor cells at doses that show no toxicity in normal counterparts. This treatment could be used as a standalone cancer therapy or in combination with other chemotherapy drugs.

Numerous in vitro studies have demonstrated the mechanism of action and efficacy. PAC-1 was tested against several cancer cell lines (including leukemia, lymphoma, melanoma, neuroblastoma, breast cancer, lung cancer, adrenal cancer, and renal cancer) that have varying concentrations of procaspase-3. Toxicity of PAC-1 was positively correlated with procaspase-3 concentration with PAC-1 showing the greatest potency against the lung cancer cell line NCI-H226 (IC50 of 0.35 M). PAC-1 has also been shown efficacious against human resected colon tumors. PAC-1 induced cell death was observed in cancer cells with IC50 values from 0.003-1.41 M versus 5.02-9.98 M in adjacent noncancerous tissue.

In vivo studies have shown PAC-1 to be efficacious in both mice and dogs. Xenograft studies of human renal and lung cancer in mouse models have demonstrated PAC-1 to reduce tumor volume relative to controls when administered via subcutaneous injection or oral gavage. No gross toxicity was observed in any of the mouse studies at low doses. S-PAC-1 is a modification of PAC-1 which displays similar in vitro results and is tolerated at much higher doses than PAC-1 (no toxicity in mice following an intravenous injection of 350 mg/kg S-PAC-1 versus motor impairments following an intravenous injection 30 mg/kg of PAC-1). Recently S-PAC-1 has been tested in dogs and no toxicity was observed after a 60 mg/kg intravenous injection. S-PAC-1 was administered as treatment for six dogs with lymphoma. S-PAC-1 was successful in reducing tumors by 27% in one dog after just four treatments, while three other dogs showed stable response.

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More information

Categories
  • Diagnosis and treatment
  • Oncology
Keywords:

programmed cell death

natural pro-death signals

small molecules capable

inactive apoptotic precursor

adjacent noncancerous tissue

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