Technologies

Knock-in mouse model with labeled insulin provides essential tool for diabetes research and drug development

Technology description

Application area

• Animal model to investigate genetic and environmental factors that cause insulin level and expression changes


• Ability to track insulin production and secretion will provide insight into effectiveness of various drug compounds for diabetes treatment


• Furtherin vivoinvestigation into insulin-secreting beta cells, which are labeled by the GFP


• The labeling of cells that interact with insulin will allow precise extraction of these cells forex vivoexperiments


• Identification of novel proteins that interact with insulin, which may serve as therapeutic targets for diabetes

Advantages

• Directly labels insulinin vivosuch that the GFP is restricted only to the cells where insulin is found


• All regulatory elements in the insulin gene are kept intact, so expression can’t be falsely altered by other local factors (like in transgenic mice)


• Any genetic or environment modifications to the endogenous insulin will be accurately represented by the GFP label