Nov. 29, 2016
Transfection of macromolecules to the cytoplasm of cells remains a challenging problem because the lipid bilayer of the cell membrane acts as a barrier to foreign molecules. However, the intracellular delivery of membrane-impermeable molecules with high efficiency and minimum side effects is a crucial process for laboratory and clinical applications. Although transfection with lipids, polymers and viral vectors is usually efficient, deliverable molecules are limited to DNA and RNA. Furthermore, cargo usually cannot be targeted to specific individual cells as desired.
USC inventors have developed an acoustic-transfection tool which uses high frequency ultrasonic pulses to remotely perturb the lipid bilayer of the cell membrane to deliver exogenous molecules into a targeted single-cell. Each ultrasonic pulse was focused into a small confined area, similar in size to a single cell, to achieve single-cell level targeting without microbubbles by an ultrasonic transducer. It is designed to deliver macromolecules with various sizes and structures into designated cells due to single-cell targeting capability. By adjusting input parameters of acoustic pulse, desired molecules for the intracellular delivery can be chosen depending on their size. After acoustic transfection, cells can be monitored by an epi-fluorescence microscope.
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minimum side effects
small confined area
adjusting input parameters
single-cell targeting capability
specific individual cells
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