Technologies

Technical Summary

Polarized epithelium bears many responsibilities including maintenance of apical and basal fluid homeostasis. These cells affect important fluid characteristics by moving a range of ions both into and out of the extracellular space using ion-specific channels, transporters, and exchangers. One such channel is the Epithelial Sodium Channel ENaC, an amiloride-sensitive heterotrimeric protein complex that contributes to sodium reabsorption in a number of major organs including the lungs, kidney, and colon. In addition to fluid regulation, ENaC has been implicated in a host of functions including taste and blood pressure maintenance, and its dysregulation can cause health complications that include severe arterial hypertension (Liddel's Syndrome) and pseudohypoaldosteronism. To facilitate further study of ENaC in a rodent model system, a rabbit polyclonal antibody has been raised to the a subunit of mouse ENaC.