Technologies

We are offering a novel kit for staining neurons and glia. Our kit, a modification of standard Golgi-Cox kits, has several advantages, as described in the table below.

Golgi-Cox impregnation has been a crucial technique for studying the morphology of neurons as well as glia. Considering the rapidly expanding scope of neuroscience that ranges from the basic research on synaptic functions, to developments of therapeutics for Alzheimer’s disease and other CNS disorders, we believe that there will be a rapidly growing demand for neuronal staining kits. Advances in computer-aided image analysis also enable use and study of the fine detail that Golgi-Cox staining provides (see images on next page). However, currently only two companies are marketing Golgi-Cox kits, and the staining procedure that these kits enable is time-consuming, limited to cortex staining, complicated, and often results in images with obvious over-staining and artifacts.

Figure 1. Comparison of standard Golgi-cox method and modified method.
Low power(4x) microscopic photograph shows difference between two methods, Standard method often cause over-crowded impregnated neurons especially in hippocampus, and over-stained neurons often cause difficulty of sampling by using Neurolucida or Camera Lucida. TH = Thalamus;
DG = Dentate Gyrus, CA = Corpus Ammonis; CTX = Cerebral Cortex.

Figure 2. Standard Golgi-Cox method leads to overstaining

Figure 3 Standard Golgi-Cox method leads to staining artifacts