Removal of Low Molecular Weight Heparin as a Treatment for Heparin Overdose

Sept. 24, 2015

Removal of Low Molecular Weight Heparin as a Treatment for Heparin Overdose

Technology description

A Technology as a Treatment for Heparin Overdose

Heparin is an anti-coagulant that is commonly used in surgery to prevent blood from clotting during procedures such as kidney dialysis, heart bypass surgery, stent implementation, indwelling catheters and knee and hip replacements. In about 5% of the cases of heparin usage, the patients experience uncontrolled bleeding which can be dangerous, especially for children. Children are usually given a particular form of heparin, low molecular weight heparin. For this form of heparin, there is currently no way to remove heparin from the blood in case of uncontrolled bleeding or heparin overdose, and the only treatment is to use an antidote, protamine sulfate. However protamine sulfate is difficult to administer properly, and causes allergic reactions in patients that are allergic to fish.

Technology:

A new material has been invented that can remove heparin from the blood without its having to actually enter the patient’s body, which is a plus for patients with weak kidney function. The new material can be placed on a cartridge such as the kind that is used in kidney dialysis, and the blood is passed over the surface of the cartridge, removing and immobilizing the heparin. For hospitals, it would be a tool that can be used in the cases of heparin overdose or uncontrolled bleeding. Heparin overdoses in adults are critical and in neonatal patients is often fatal. This new material can save lives.

The following link provides more information on the technology, and the need for it in the marketplace.

http://vpred.uark.edu/documents/techventures/tech_docs/heparin_market_report.pdf

Application area

* As an affinity tag for simple and rapid purification of recombinant proteins expressed in different expression hosts.
* As a cell/molecular biology tool to quantitatively detect the expression levels of heparin binding proteins cells/tissues under different experimental conditions.
* Neutralization of the anticoagulant activity of heparin during cardiovascular surgeries.
* As an antiangiogenic agent to inhibit tumor metastases induced by growth factors such as, the vascular endothelial growth factor and the fibroblast growth factors.
* For the purification of heparin and other glycosaminoglycans.
* As a diagnostic marker to identify patients at risk of developing sepsis with circulatory failure.
* As an antimicrobial agent against both gram positive and gram negative bacteria.
* As an inhibitor against herpesvirus and dengue virus infections.
* As an antifungal agent
*The invention is available for licensing.

Advantages

* The HB-tag facilitates rapid, reproducible and single-step purification of recombinant proteins overexpressed in different expression hosts.
* Purification of HB-fused recombinant proteins can be achieved using common salt (NaCl).
* Target protein can be easily released from the HB-tag by proteolytic cleavage.
* HB-fused recombinant proteins can be purified even in the presence of chemical denaturants.
* HB-fused recombinant proteins can be quantitatively detected using polyclonal antibodies.
* HB-tag can be used to purify glycosaminoglycans.
* HB-tag has potential pharmaceutical applications

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