Technologies

说明

Members of the genus Meloidogyne (Chitwood; over 90 species), the root-knot nematodes (roundworm), are an agronomically important group of pathogens that establish a complex parasitic relationship with a wide range of host plants. Currently, well over 2000 plants are susceptible to root-knot nematode infections, leading to over 5% of global crop loss. Substances produced in the esophageal glands of Chitwood are secreted through the stylets of feeding root-knot nematodes, modifying several root protophloem cells into elaborate feeding sites called giant-cells. Nutrients from the giant-cells are required for the growth and pronounced morphological changes that occur during nematode development from a motile, vermiform juvenile to an enlarged, rounded, sedentary adult female. These changes include a reduction in size of the two subventral esophageal gland cells and an increase in size of the single dorsal esophageal gland cell as the nematode develops to an adult female. The development of monoclonal antibodies may help elucidate the function of several nematodal structures in organismal development and plant parasitism by facilitating isolation of proteins from complex mixtures and aid in the development of counter-measures. All nematodes pass through an embryonic stage, four juvenile stages (J1–J4) and an adult stage. Given certain similarities of Mab binding across multiple species of the Nematoda phylum, these antibodies may also be suitable for the study of development of, and parasitism by, other members of the phylum.

Reagents Description

References

Davis EL, Aron, LM, Pratt, LH, Hussey, RS: Novel immunization Procedures used to Develop Monoclonal Antibodies that Bind to Specific Structures inMeloidogyne spp.(1992). Phytopathology, 82(10), 1244-1250 .

Hussey, R.S.: Monoclonal Antibodies to Secretory Granules in Esophageal Glands inMeloidogyneSpecies. (1989) J. Nematology, 21(3), 392-398

Ray, C, Abbott AG, Hussey, RS: Trans-splicing of aMeloidogyne incognitamRNA encoding a putative esophageal gland protein. (1994). Mol. Biochem. Parasitology , 68, 93-101

Hussey, R. S. & Grundler, F. M. W. 1998 Nematode parasitism of plants. In: The Physiology and Biochemistry of free-living and plant-parasitic nematodes . Perry, R. N. & Wright, D. J. (Eds), CABI Publishing, UK. pp 213 – 243

Hussey, RS, Paguio, OR, Seabury, F: (1990) Localization and purification of a secretory protein from the esophageal glands ofMeloidogyne incognitawith a monoclonal antibody. Phytopathology, 80, 709-714