Technologies

Cyclin E is a member of the cyclin family of proteins, which regulates the cell cycle through its activation of cyclin-dependent kinases. Specifically, cyclin E binds and activates the S phase Cdk2. The cyclin E–Cdk2 complex promotes the G1 to S phase cell cycle transition. Overexpression of cyclin E has been implicated in carcinomas among the gastrointestinal tract, including colon or stomach cancer as well as a marker for breast cancers.

Reagent Description

Antigen: rec-C. elegansCYE-1

Accession ID: NM_001025857.2

Molecular Weight: 60.6 kDa

Clone Name: 17C8

Reactivity: C. elegans / C. briggsae

Immunogen: Full-length recombinant CYE-1 protein

Species Immunized: Mouse

Purification Method: Not purified; used as antibody supernatant

Tested Applications: WB, IF.

MAb storage: -80 ^o C

References

Brodigan, T.M., Liu, J., Park, M., Kipreos, E.T., and Krause, M. 2003. Cyclin E expression during development in C. elegans. Developmental Biology 254: 102-115 .

Kim, Y., and Kipreos, E.T. 2007. The C. elegans replication licensing factor CDT-1 is targeted for degradation by the CUL-4/DDB-1 complex. Molecular and Cellular Biology 27: 1394-1406 .

The, I., Ruijtenberg, S., Bouchet, B. P., Cristobal, A., Prinsen, M. B., van Mourik, T., Koreth, J., Xu, H., Heck, A. J., Akhmanova, A., Cuppen, E., Boxem, M., Munoz, J., & van den Heuvel, S. (2015). Rb and FZR1/Cdh1 determine CDK4/6-cyclin D requirement in C. elegans and human cancer cells. Nat Commun, 6, 5906 .

Fox, P. M., Vought, V. E., Hanazawa, M., Lee, M. H., Maine, E. M., & Schedl, T. (2011). Cyclin E and CDK-2 regulate proliferative cell fate and cell cycle progression in the C. elegans germline. Development, 138 , 2223-34 .