Technologies

time icon Feb. 1, 2012

Microwave-Assisted Freeze Substitution of Biological and Biomedical Samples (MWFS)

Technology description

Freeze substitution fixation (FS) of hydrated samples frozen in vitreous ice provides exceptional preservation of structure for light and electron microscopy, and enables immunological detection of thermo-labile antigens that otherwise are damaged/destroyed by processing at ambient or elevated temperatures. Its use as a research tool or in clinical pathology has, however, been limited by the relatively lengthy periods required for passive diffusion of fixatives and organic solvents into the frozen hydrated material.
The instant invention utilizes controlled microwave (MW) irradiation to accelerate the FS process; and comprises systems, devices and methods for microwave-assisted processing of samples under cryo-conditions. The entire MWFS procedure has been accomplished in less than 4 hours as compared to the approximately 2-5 days required for FS.

Application area

  • Provides superior preservation and rapid turnaround in research and high throughput clinical laboratory settings
  • Applicable to a broad range of biological samples, hydrogels, and other hydrated materials
  • Processing for light and electron microscopy
  • Low-temperature synthetic and analytical chemistry

Advantages

  • Reduces processing periods from days to hours
  • Improves preservation, approaching native state
  • Enables uncomplicated, programmable operation
  • Provides excellent reproducibility

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More information

Categories
  • Pathology
  • Diagnosis and treatment
Keywords:

freeze substitution fixation

enables immunological detection

lengthy periods required

frozen hydrated material

entire mwfs procedure

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