Technologies

Technical Details:
Hypoxia-induced mitogenic factor (HIMF) is highly up regulated in the lungs as a result of hypoxia-induced pulmonary hypertension, and has proangiogenic and proinflammatory effects in the lung. As a research tool for studying the function and activity of HIMF, JHU researchers have developed a transgenic HEK 293 cell line expresses a Flag-tagged HIMF. Expression of the Flag-tagged HIMF protein can be induced by tetracycline and most of the protein is secreted into the culture medium where it can be readily purified by column chromatography.